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- 產(chǎn)品名稱： RalA Activation Assay Kit
- 產(chǎn)品貨號(hào)： 83601
- 貨期： 現(xiàn)貨
- 價(jià)格與訂購(gòu)： 6800
- 數(shù)量：
庫(kù)存： 5
- 規(guī)格： 96T
- 產(chǎn)品信息
- 如何訂購(gòu)
Product Description
Small GTPases are a super-family of cellular signaling regulators. Sar1 is a member of these smallGTPases. Sar1 functions as a molecular switch to control protein–protein and protein–lipid interactions that direct vesicle budding from the ER. Sar1 is invloved in membrane trafficking, andregulates the assembly and disassembly of COPII coats.Currently there is no direct assay to measure the activation of Sar1 GTPases.
Bioyears Biosciences Sar1 Activation Assay Kit is based on the configuration-specific monoclonalantibody that specifically recognizes Sar1 GTP, but not Sar1 GDP. Given the high affinity ofmonoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility
These anti Sar1 GTP monoclonal antibodies can also be used to monitor the activation of Sar1 in cellsand in tissues by immunohistochemistry.
Bioyears Biosciences Sar1 Activation Assay Kit provides a simple and fast method to monitor the activation of Sar1. Each kit provides sufficient quantities to perform 20 assays.
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Assay Principle
Bioyears Biosciences RalA Activation Assay Kit bases on the configuration-specific anti Ral GTP monoclonal antibody to measure the active Ral-GTP levels, either from cell extracts or from in vitro GTPγS loading Ral activation assays. Briefly, anti active Ral mouse monoclonal antibody will be incubated with cell lysates containing Ral-GTP. The bound active Ral will then be pulled down by protein A/G agarose. The precipitated active RalB or RalA will be detected by immunoblot analysis using anti RalB or RalA rabbit polyclonal antibody, respectively.
Kit Components
1. Anti active Ral, Mouse Monoclonal Antibody (Catalog No. 26913): One vial – 22 μL (1
mg/ml) in PBS, pH 7.4, containing 50% glycerol and 0.05% sodium azide. This antibody
specifically recognizes Ral-GTP from all vertebrates.
2. Protein A/G Agarose (Catalog No. 30301): One vial – 400 μL of 50% slurry.
3. 5X Assay/Lysis Buffer (Catalog No. 30303): One bottle – 30 mL of 250 mM Tris-HCl, pH 8, 750 mM NaCl, 50 mM MgCl2, 5 mM EDTA, 5% Triton X-100.
4. Anti RalA, Rabbit Polyclonal Antibody (Catalog No. 21034): One vial – 100 μL (1 mg/ml) in
PBS, pH 7.4, contained 50% glycerol.
5. 100 X GTPγS (Catalog No. 30302): One vial –100 μl at 10 mM, use 5 μL of GTPγS for
GTP-labeling of 0.5 mL of cell lysate.
6. 100 X GDP (Catalog No. 30304): One vial –100 μl at 100 mM, use 5 μL of GDP for
GDP-labeling of 0.5 mL of cell lysate.
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Storage
Store all kit components at 4oC until their expiration dates.
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Note
For Research Use Only.
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